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C20orf20 Polyclonal antibody

C20orf20 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 26040-1-AP

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Synonyms

C20orf20, Eaf7, MRG binding protein, MRG15BP, MRGBP, URCC4



经过测试的应用

Positive WB detected inHepG2 cells, mouse testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26040-1-AP is C20orf20 Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen C20orf20 fusion protein Ag22840 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 20 open reading frame 20
Synonyms C20orf20, Eaf7, MRG binding protein, MRG15BP, MRGBP, URCC4
Calculated Molecular Weight 204 aa, 22 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC014235
Gene Symbol C20orf20
Gene ID (NCBI) 55257
RRIDAB_2880347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV56
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C20orf20, also named as MRG/MORF4L-binding protein or Up-regulated in colon cancer 4 (Urcc4), is a 204 amino acid protein, which belongs to the EAF7 family. C20orf20 localizes in the nucleus and is a Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. C20orf20 is modified through phosphorylation and acetylation, so the molecular weight of C20orf20 might be 25-30 kDa.

实验方案

Product Specific Protocols
WB protocol for C20orf20 antibody 26040-1-APDownload protocol
IHC protocol for C20orf20 antibody 26040-1-APDownload protocol
IF protocol for C20orf20 antibody 26040-1-APDownload protocol
IP protocol for C20orf20 antibody 26040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

MRGBP promotes AR-mediated transactivation of KLK3 and TMPRSS2 via acetylation of histone H2A.Z in prostate cancer cells.

Authors - Saya Ito
  • KD Validated