CA2 Polyclonal antibody

CA2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16961-1-AP

Print datasheet

Synonyms

CA II, CA2, CAC, CAII, Car2, Carbonate dehydratase II, Carbonic anhydrase 2, Carbonic anhydrase C, carbonic anhydrase II



经过测试的应用

Positive WB detected inHEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells
Positive IP detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16961-1-AP targets CA2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CA2 fusion protein Ag10680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonic anhydrase II
Synonyms CA II, CA2, CAC, CAII, Car2, Carbonate dehydratase II, Carbonic anhydrase 2, Carbonic anhydrase C, carbonic anhydrase II
Calculated Molecular Weight 260 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC011949
Gene Symbol CA2
Gene ID (NCBI) 760
RRIDAB_2065857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00918
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA2(carbonic anhydrase II ) is also named as Car2, CA-II, CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in the development of brain(PMID:16825953). Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3).

实验方案

Product Specific Protocols
WB protocol for CA2 antibody 16961-1-APDownload protocol
IP protocol for CA2 antibody 16961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.

Authors - Khositseth Sookkasem S
humanWB,IHC

Sci Rep

SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma.

Authors - Yanzhang Luo
humanWB

J Inflamm Res

M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

Authors - Xianqin Song
mouseWB

Front Mol Neurosci

Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.

Authors - Kalina Wiatr
humanWB

J Cancer

Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network.

Authors - Wanpeng Wang
WB

J Physiol

Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.

Authors - Jeong Hee Hong