- Featured Product
- KD/KO Validated
c-Cbl Monoclonal antibody
c-Cbl Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2F6D1
验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, Daudi cells, K-562 cells, Jurkat cells, THP-1 cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
66576-1-Ig targets c-Cbl in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | c-Cbl fusion protein Ag23033 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Cas-Br-M (murine) ecotropic retroviral transforming sequence |
| Synonyms | C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55 |
| Calculated Molecular Weight | 906 aa, 100 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | BC132733 |
| Gene Symbol | CBL |
| Gene ID (NCBI) | 867 |
| RRID | AB_2881936 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| IHC protocol for c-Cbl antibody 66576-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy RAB26-dependent autophagy protects adherens junctional integrity in acute lung injury.
| ||
