Cyclin D2 Monoclonal antibody

Cyclin D2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1A12A8

Cat No : 67048-1-Ig

Print datasheet

Synonyms

CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002



经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, U2OS cells, MCF-7 cells, Caco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

67048-1-Ig targets Cyclin D2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,ELISA
Cited Applications WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen Cyclin D2 fusion protein Ag25190 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin D2
Synonyms CCND2, cyclin D, cyclin D2, G1/S specific cyclin D2, KIAK0002
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC010958
Gene Symbol CCND2
Gene ID (NCBI) 894
RRIDAB_2882361
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCND2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. CCND2 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. High level expression of CCND2 was observed in ovarian and testicular tumors.

实验方案

Product Specific Protocols
WB protocol for Cyclin D2 antibody 67048-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation

Authors - Cunying Ma
humanWB

Technol Cancer Res Treat

The Analysis of Differentially Expressed circRNAs Under the Antiproliferative Effect From 5-Fluorouracil on Osteosarcoma Cells.

Authors - AiJun Huang
humanWB

Cell Death Dis

Long intergenic non-coding RNA 00473 promotes proliferation and migration of gastric cancer via the miR-16-5p/CCND2 axis and by regulating AQP3.

Authors - Shuaishuai Zhuo
human,mouseWB

Am J Cancer Res

miR-1297 inhibits osteosarcoma cell proliferation and growth by targeting CCND2

Authors - Pan Jiang
humanWB

Int Immunopharmacol

Hydroxypropyl-β-cyclodextrin inhibits the development of triple negative breast cancer by enhancing antitumor immunity

Authors - Mengmeng Zhu