MME,CD10 Polyclonal antibody

MME,CD10 Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF, IHC, ELISA

Conjugate

Unconjugated

Cat No : 23782-1-AP

Print datasheet

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11



经过测试的应用

Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23782-1-AP targets MME,CD10 in IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, ELISA
Tested Reactivity human
Immunogen MME,CD10 fusion protein Ag20634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane metallo-endopeptidase
Synonyms Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11
Calculated Molecular Weight 750 aa, 86 kDa
GenBank Accession NumberBC101632
Gene Symbol MME
Gene ID (NCBI) 4311
RRIDAB_2879323
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

实验方案

Product Specific Protocols
IHC protocol for MME,CD10 antibody 23782-1-APDownload protocol
IF protocol for MME,CD10 antibody 23782-1-APDownload protocol
FC protocol for MME,CD10 antibody 23782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols