CD107a / LAMP1 Monoclonal antibody

CD107a / LAMP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B2E9

Cat No : 67300-1-Ig

Print datasheet

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker



经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, Jurkat cells, THP-1 cells, MDA-MB-231 cells, JAR cells, K-562 cells, U-937 cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue

This antibody is not recommended for immunocytofluorescent assays.

Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67300-1-Ig targets CD107a / LAMP1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD107a / LAMP1 fusion protein Ag28869 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 90-120 kDa
GenBank Accession NumberBC021288
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_2882564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

实验方案

Product Specific Protocols
WB protocol for CD107a / LAMP1 antibody 67300-1-IgDownload protocol
IHC protocol for CD107a / LAMP1 antibody 67300-1-IgDownload protocol
IF protocol for CD107a / LAMP1 antibody 67300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Generation and Characterization of SORT1-Targeted Antibody-Drug Conjugate for the Treatment of SORT1-Positive Breast Tumor

Authors - Weiliang Zhuang
humanWB,IF

Cancers (Basel)

A Novel Phenazine Analog, CPUL1, Suppresses Autophagic Flux and Proliferation in Hepatocellular Carcinoma: Insight from Integrated Transcriptomic and Metabolomic Analysis

Authors - Jiaqin Chen
mouseWB

MedComm (2020)

Leucine-rich repeat kinase 2 (LRRK2) inhibition upregulates microtubule-associated protein 1B to ameliorate lysosomal dysfunction and parkinsonism

Authors - Kang Chen
IF

J Nanobiotechnology

A novel "prime and pull" strategy mediated by the combination of two dendritic cell-targeting designs induced protective lung tissue-resident memory T cells against H1N1 influenza virus challenge

Authors - Zhannan Wang
mouseWB

Antioxidants (Basel)

Stimulation of Hepatic Ferritinophagy Mitigates Irp2 Depletion-Induced Anemia

Authors - Yutong Liu
IF

Nat Commun

Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy

Authors - Lin Li