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CoraLite® Plus 488 Anti-Mouse CD127 (IL-7Ra) (A7R34)

CD127 (IL-7Ra) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

A7R34

Cat No : CL488-65093

Print datasheet

Synonyms

CD127, CD127 (IL-7Ra), IL 7Ralpha, Il7r, interleukin 7 receptor

验证数据展示

  • FC experiment of mouse splenocytes using CL488-65093

    FC experiment of mouse splenocytes using CL488-65093

    1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 1 ug CoraLite® Plus 488 Anti-Mouse CD127 (IL-7Ra) (CL488-65093, Clone: A7R34) or 1 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65093-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65093 targets CD127 (IL-7Ra) in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen IL-7Ra-IgG1 fusion protein 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name interleukin 7 receptor
Synonyms CD127, CD127 (IL-7Ra), IL 7Ralpha, Il7r, interleukin 7 receptor
GenBank Accession NumberBC089571
Gene Symbol Il7r
Gene ID (NCBI) 16197
RRIDAB_2883173
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP16872
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD127 (IL-7Ra) antibody CL488-65093Download protocol
Standard Protocols
Click here to view our Standard Protocols