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Anti-Mouse CD134 (OX-86)

CD134 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

OX-86

Cat No : 65136-1-Ig

Print datasheet

Synonyms

ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L

验证数据展示

  • FC experiment of BALB/c mouse splenocytes using 65136-1-Ig

    FC experiment of BALB/c mouse splenocytes using 65136-1-Ig

    1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2), 1 ug Anti-Mouse CD134 (65136-1-Ig, Clone: OX-86) or rat IgG1 isotype control, and FITC anti-rat IgG1 Antibody at dilution 1:100. Cells were not fixed.

经过测试的应用

Positive FC detected inCon-A stimulated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65136-1-Ig targets CD134 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen N/A 种属同源性预测
Host / Isotype Rat / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 4
Synonyms ACT35, CD134, Ly 70, Ox40, Tnfrsf4, Txgp1, TXGP1L
GenBank Accession NumberBC065782
Gene Symbol Tnfrsf4
Gene ID (NCBI) 22163
RRIDAB_2918423
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

实验方案

Product Specific Protocols
FC protocol for CD134 antibody 65136-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols