CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IF, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4F3A8

Cat No : 67155-1-Ig

Print datasheet

Synonyms

CD138, SDC, SDC1, SYND1, syndecan, syndecan 1



经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human ovary tumor tissue, human appendicitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

67155-1-Ig targets CD138/Syndecan-1 in IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC,ELISA
Cited Applications IF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name syndecan 1
Synonyms CD138, SDC, SDC1, SYND1, syndecan, syndecan 1
Calculated Molecular Weight 32.5 kDa
GenBank Accession NumberBC008765
Gene Symbol SDC1
Gene ID (NCBI) 6382
RRIDAB_2882452
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18827
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

实验方案

Product Specific Protocols
IHC protocol for CD138/Syndecan-1 antibody 67155-1-IgDownload protocol
IF protocol for CD138/Syndecan-1 antibody 67155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanIF

Nat Commun

Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection

Authors - Pei-Fen Zhang