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CoraLite® Plus 488 Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

TS1/18

Cat No : CL488-65190

Print datasheet

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL488-65190

    FC experiment of human peripheral blood lymphocytes using CL488-65190

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul/test CoraLite® Plus 488-conjugated Anti-Human CD18 (CL488-65190, Clone: TS1/18) (red) or isotype control antibody (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65190-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65190 targets CD18 in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen Human Beta 2 Integrin 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Synonyms CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7
Calculated Molecular Weight 85 kDa
GenBank Accession NumberBC005861
Gene Symbol ITGB2
Gene ID (NCBI) 3689
ENSEMBL Gene IDENSG00000160255
RRIDAB_2883213
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05107
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD18 antibody CL488-65190Download protocol
Standard Protocols
Click here to view our Standard Protocols