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CD23 Polyclonal antibody

CD23 Polyclonal Antibody for WB,IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

Cat No : 18642-1-AP

Print datasheet

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

验证数据展示

  • WB analysis of Raji using 18642-1-AP

    WB analysis of Raji using 18642-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 18642-1-AP

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CD23 antibody included
  • IHC staining of human tonsillitis using 18642-1-AP

    IHC staining of human tonsillitis using 18642-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18642-1-AP (FCER2,CD23 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CD23 antibody included

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18642-1-AP targets CD23 in WB,IHC,ELISA applications and shows reactivity with human samples.

Tested Applications WB,IHC,ELISA
Tested Reactivity human
Immunogen CD23 fusion protein Ag13348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Synonyms BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC014108
Gene Symbol FCER2
Gene ID (NCBI) 2208
RRIDAB_2878555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

实验方案

Product Specific Protocols
WB protocol for CD23 antibody 18642-1-APDownload protocol
IHC protocol for CD23 antibody 18642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols