CoraLite® Plus 488-conjugated CD3 Gamma Monoclonal antibody
CD3 Gamma Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2E9G7
验证数据展示
经过测试的应用
| Positive IF detected in | human tonsillitis tissue |
For other applications, we recommend the unconjugated version of this antibody, 60347-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-60347 targets CD3 Gamma in IF applications and shows reactivity with human samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | human |
| Immunogen | CD3 Gamma fusion protein Ag15502 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD3g molecule, gamma (CD3-TCR complex) |
| Synonyms | CD3 GAMMA, CD3G, T cell receptor T3 gamma chain, T3G |
| Calculated Molecular Weight | 182 aa, 20 kDa |
| GenBank Accession Number | BC113830 |
| Gene Symbol | CD3G |
| Gene ID (NCBI) | 917 |
| RRID | AB_2883146 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09693 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD3 Gamma antibody CL488-60347 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |