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Anti-Mouse CD54 (YN1/1.7.4)

CD54 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

YN1/1.7.4

Cat No : 65066-1-Ig

Print datasheet

Synonyms

CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2

验证数据展示

  • FC experiment of mouse splenocytes using 65066-1-Ig

    FC experiment of mouse splenocytes using 65066-1-Ig

    1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD54 (65066-1-Ig, Clone: YN1/1.7.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (green), or stained with 0.5 ug Rat IgG2b Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:200 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65066-1-Ig targets CD54 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Mouse NS-1 cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM 1, Icam1, ICAM-1, Ly 47, MALA 2
GenBank Accession NumberBC008626
Gene Symbol Icam1
Gene ID (NCBI) 15894
RRIDAB_2918373
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP13597
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for CD54 antibody 65066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols