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CD57 Monoclonal antibody

CD57 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F4A11

Cat No : 66711-1-Ig

Print datasheet

Synonyms

B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1

验证数据展示

  • WB analysis using 66711-1-Ig

    WB analysis using 66711-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (CD57 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 66711-1-Ig

    WB analysis of mouse brain using 66711-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (CD57 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 66711-1-Ig

    FC experiment of K-562 using 66711-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inK-562 cells, mouse brain tissue, Jurkat cells, PC-3 cells, SH-SY5Y cells, U-251 cells
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66711-1-Ig targets CD57 in WB, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications FC, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen CD57 fusion protein Ag13593 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Synonyms B3GAT1, CD57, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa, 100 kDa
GenBank Accession NumberBC010466
Gene Symbol B3GAT1
Gene ID (NCBI) 27087
RRIDAB_2882062
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P2W7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

实验方案

Product Specific Protocols
WB protocol for CD57 antibody 66711-1-IgDownload protocol
FC protocol for CD57 antibody 66711-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols