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CDC23 Polyclonal antibody

CDC23 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 10683-1-AP

Print datasheet

Synonyms

ANAPC8, APC8, CDC23, Cyclosome subunit 8

验证数据展示

  • WB analysis using 10683-1-AP

    WB analysis using 10683-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10683-1-AP (CDC23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMOLT-4 cells, HEK-293 cells, Jurkat cells, K-562 cells, Caco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10683-1-AP targets CDC23 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen CDC23 fusion protein Ag1068 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 23 homolog (S. cerevisiae)
Synonyms ANAPC8, APC8, CDC23, Cyclosome subunit 8
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC005258
Gene Symbol CDC23
Gene ID (NCBI) 8697
RRIDAB_2075008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJX2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC23(cell division cycle protein 23 homolog) is also named ANAPC8 and belongs to the APC8/CDC23 family. It is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. It can be phosphorylated and the phosphorylation of Thr-562 occurs specifically during mitosis(PMID:19690332). It has 3 isoforms produced by alternative splicing with the molecular weight of 69 kDa, 17 kDa, and 56 kDa.

实验方案

Product Specific Protocols
WB protocol for CDC23 antibody 10683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols