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CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

FC, IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F10G2

Cat No : 67239-1-Ig

Print datasheet

Synonyms

ANAPC3, APC3, CDC27, CDC27 homolog, CDC27; APC3, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC

验证数据展示

  • IHC staining of human lung cancer using 67239-1-Ig

    IHC staining of human lung cancer using 67239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67239-1-Ig

    IHC staining of human lung cancer using 67239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 67239-1-Ig

    FC experiment of K-562 using 67239-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

  • WB analysis of Jurkat using 67239-1-Ig

    WB analysis of Jurkat using 67239-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67239-1-Ig

    IF Staining of HEK-293 using 67239-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, human lung cancer tissue
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67239-1-Ig targets CDC27; APC3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC, IF/ICC,IF-P, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen CDC27; APC3 fusion protein Ag25588 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cell division cycle 27 homolog (S. cerevisiae)
Synonyms ANAPC3, APC3, CDC27, CDC27 homolog, CDC27; APC3, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC011656
Gene Symbol CDC27
Gene ID (NCBI) 996
RRIDAB_2882518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

实验方案

Product Specific Protocols
WB protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
IHC protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
IF protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
FC protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols