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  • Featured Product
  • KD/KO Validated

HRPT2, CDC73 Polyclonal antibody

HRPT2, CDC73 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12310-1-AP

Print datasheet

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, Hyperparathyroidism 2 protein, Parafibromin

验证数据展示

  • WB analysis of HEK-293 using 12310-1-AP

    WB analysis of HEK-293 using 12310-1-AP

    WB result of HRPT2, CDC73 antibody (12310-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HEK-293 cells.

  • WB analysis using 12310-1-AP

    WB analysis using 12310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12310-1-AP

    IHC staining of human breast cancer using 12310-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC73 antibody included
  • IHC staining of human breast cancer using 12310-1-AP

    IHC staining of human breast cancer using 12310-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC73 antibody included
  • IF Staining of HeLa using 12310-1-AP

    IF Staining of HeLa using 12310-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRPT2, CDC73 antibody (12310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IP experiment of mouse testis using 12310-1-AP

    IP experiment of mouse testis using 12310-1-AP

    IP result of anti-HRPT2, CDC73 (IP:12310-1-AP, 4ug; Detection:12310-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDC73 Ready-To-Use IHC Kit. CDC73 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12310-1-AP targets HRPT2, CDC73 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HRPT2, CDC73 fusion protein Ag2959 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Synonyms C1orf28, CDC73, HPT JT, HRPT2, Hyperparathyroidism 2 protein, Parafibromin
Calculated Molecular Weight 531 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014351
Gene Symbol CDC73
Gene ID (NCBI) 79577
RRIDAB_2078388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P1J9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

实验方案

Product Specific Protocols
WB protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IHC protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IF protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IP protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao
View Article