P-cadherin Polyclonal antibody

P-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13773-1-AP

Print datasheet

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, PCAD, P-cadherin



经过测试的应用

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13773-1-AP targets P-cadherin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen P-cadherin fusion protein Ag4758 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cadherin 3, type 1, P-cadherin (placental)
Synonyms Cadherin 3, CDH3, CDHP, HJMD, PCAD, P-cadherin
Calculated Molecular Weight 829 aa, 91 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC041846
Gene Symbol CDH3
Gene ID (NCBI) 1001
RRIDAB_10596468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22223
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

实验方案

Product Specific Protocols
WB protocol for P-cadherin antibody 13773-1-APDownload protocol
IHC protocol for P-cadherin antibody 13773-1-APDownload protocol
IF protocol for P-cadherin antibody 13773-1-APDownload protocol
IP protocol for P-cadherin antibody 13773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.

Authors - Hans I-Chen Harn
mouseIF

Mol Ther Nucleic Acids

Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.

Authors - Ziyang Wang
humanIHC

Aging (Albany NY)

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Authors - Jing Han
humanWB,IHC

Front Oncol

Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.

Authors - Meng-Jie Shan
humanWB,IHC

Int J Biol Sci

KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.

Authors - Li Li
mouseIF

Dev Dyn

Denticleless E3 ubiquitin protein ligase (DTL) maintains the proliferation and differentiation of epidermis and hair follicles during skin development

Authors - Yanhui Lin