• Featured Product
  • KD/KO Validated

CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11517-1-AP

Print datasheet

Synonyms

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAP, LIP1, LYST interacting protein 1, MCPH6



经过测试的应用

Positive WB detected inmouse testis tissue, Jurkat cells
Positive IF detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11517-1-AP targets CENPJ in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen CENPJ fusion protein Ag2078 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centromere protein J
Synonyms BM032, CENP J, CENPJ, centromere protein J, CPAP, LAP, LIP1, LYST interacting protein 1, MCPH6
Calculated Molecular Weight 1338 aa, 153 kDa
Observed Molecular Weight 153 kDa
GenBank Accession NumberBC024209
Gene Symbol CENPJ
Gene ID (NCBI) 55835
RRIDAB_2244605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

实验方案

Product Specific Protocols
WB protocol for CENPJ antibody 11517-1-APDownload protocol
IF protocol for CENPJ antibody 11517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.

Authors - Franz Meitinger
humanIF

Science

Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.

Authors - Yao Liang Wong
mouseWB,IF

Nat Cell Biol

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Authors - Huijie Zhao
mouseWB,IF

Neuron

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Authors - Divya Jayaraman
ratIF

Dev Cell

SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication.

Authors - Chii Shyang Fong
humanIF

Nat Commun

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.

Authors - Søs Grønbæk Holdgaard