Chordin Polyclonal antibody

Chordin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27293-1-AP

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Synonyms

chordin, CHRD



经过测试的应用

Positive WB detected in3T3-L1 cells
Positive IHC detected inhuman liver cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27293-1-AP targets Chordin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Chordin fusion protein Ag26001 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chordin
Synonyms chordin, CHRD
Calculated Molecular Weight 955 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC112345
Gene Symbol CHRD
Gene ID (NCBI) 8646
RRIDAB_2880832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2X0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. CHRD may also have roles in organogenesis and during adulthood. It has been suggested that CHRD could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of CHRD results in enhanced bone regeneration.

实验方案

Product Specific Protocols
WB protocol for Chordin antibody 27293-1-APDownload protocol
IHC protocol for Chordin antibody 27293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols