COPS8/COP9 Polyclonal antibody
COPS8/COP9 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, SH-SY5Y cells, PC-12 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human colon cancer tissue, mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
产品信息
10089-2-AP targets COPS8/COP9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | COPS8/COP9 fusion protein Ag0143 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis) |
Synonyms | COP9, COP9 homolog, COPS8, COPS8/COP9, CSN8, hCOP9, SGN8, Signalosome subunit 8 |
Calculated Molecular Weight | 23 kDa |
Observed Molecular Weight | 23 kDa |
GenBank Accession Number | BC003090 |
Gene Symbol | COPS8 |
Gene ID (NCBI) | 10920 |
RRID | AB_2081903 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q99627 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for COPS8/COP9 antibody 10089-2-AP | Download protocol |
IHC protocol for COPS8/COP9 antibody 10089-2-AP | Download protocol |
IP protocol for COPS8/COP9 antibody 10089-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Bioeng Biotechnol Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts. | ||
Biochem Biophys Res Commun Rig-G negatively regulates SCF-E3 ligase activities by disrupting the assembly of COP9 signalosome complex. | ||
Neuropsychiatr Dis Treat COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. |