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COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27308-1-AP

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Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2



经过测试的应用

Positive WB detected inA549 cells, mouse lung tissue
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27308-1-AP targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Synonyms COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2
Calculated Molecular Weight 604 aa, 68 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC013734
Gene Symbol PTGS2
Gene ID (NCBI) 5743
RRIDAB_2880838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35354
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

实验方案

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
WB

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
mouseWB,IF

ACS Appl Mater Interfaces

Programmable Polymeric Microneedles for Combined Chemotherapy and Antioxidative Treatment of Rheumatoid Arthritis.

Authors - Chaoxiong Wu
mouseIHC

Redox Biol

Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin.

Authors - Jana Jandova
mouseWB,IHC

J Control Release

Combination of mitochondria impairment and inflammation blockade to combat metastasis

Authors - Xiaoli Yi
humanWB

Free Radic Biol Med

PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis

Authors - Qiong Sun