Ceruloplasmin Polyclonal antibody
Ceruloplasmin Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human blood tissue, mouse brain tissue, human plasma tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy Ceruloplasmin/CP Ready-To-Use IHC Kit. Ceruloplasmin/CP primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 21131-1-AP is Ceruloplasmin Fusion Protein expressed in E. coli.
| Tested Applications | IHC, WB,ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Ceruloplasmin fusion protein Ag15535 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ceruloplasmin (ferroxidase) |
| Synonyms | Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase |
| Calculated Molecular Weight | 1065 aa, 122 kDa |
| Observed Molecular Weight | 130-140 kDa, 85 kDa |
| GenBank Accession Number | BC146663 |
| Gene Symbol | CP |
| Gene ID (NCBI) | 1356 |
| RRID | AB_2878815 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00450 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Ceruloplasmin antibody 21131-1-AP | Download protocol |
| IHC protocol for Ceruloplasmin antibody 21131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cells Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis | ||
J Clin Pharmacol Expression of ceruloplasmin in the peripheral blood of patients with drug-resistant epilepsy | ||
Cell Death Dis The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice | ||
iScience Hepatic depletion of nucleolar protein mDEF causes excessive mitochondrial copper accumulation associated with p53 and NRF1 activation | ||
Mol Neurobiol Gemfibrozil Alleviates Cognitive Impairment by Inhibiting Ferroptosis of Astrocytes via Restoring the Iron Metabolism and Promoting Antioxidant Capacity in Type 2 Diabetes |
