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CSE1L Monoclonal antibody

CSE1L Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1A11

Cat No : 67306-1-Ig

Print datasheet

Synonyms

CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2

验证数据展示

  • WB analysis using 67306-1-Ig

    WB analysis using 67306-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67306-1-Ig (CSE1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67306-1-Ig

    IHC staining of human colon cancer using 67306-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67306-1-Ig (CSE1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSE1L antibody included
  • IHC staining of human colon cancer using 67306-1-Ig

    IHC staining of human colon cancer using 67306-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67306-1-Ig (CSE1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSE1L antibody included
  • IF Staining of human colon cancer using 67306-1-Ig

    IF Staining of human colon cancer using 67306-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CSE1L antibody (67306-1-Ig, Clone: 1G1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 67306-1-Ig

    IF Staining of human colon cancer using 67306-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CSE1L antibody (67306-1-Ig, Clone: 1G1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells, Jurkat cells, HepG2 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy CSE1L Ready-To-Use IHC Kit. CSE1L primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67306-1-Ig targets CSE1L in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen CSE1L fusion protein Ag29131 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CSE1 chromosome segregation 1-like (yeast)
Synonyms CAS, CSE1, CSE1L, Exp2, Exportin 2, Importin alpha re exporter, XPO2
Calculated Molecular Weight 971 aa, 110 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC109313
Gene Symbol CSE1L
Gene ID (NCBI) 1434
RRIDAB_2882568
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55060
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSE1L antibody 67306-1-IgDownload protocol
IHC protocol for CSE1L antibody 67306-1-IgDownload protocol
IF protocol for CSE1L antibody 67306-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols