Gamma cystathionase Antibody 64 Publications

Rabbit Polyclonal| Catalog number: 12217-1-AP

Featured Product KD/KO validated

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规格

单价

50 μl

¥980

100 μl

¥1800

150 μl

¥2500


Species specificity:
human, mouse, rat, Rabbit

Positive WB detected in:
mouse kidney tissue, mouse heart tissue, rat heart tissue, HeLa cells, rat liver tissue, HepG2 cells

Positive IP detected in:
HEK-293 cells, mouse liver tissue

Positive IHC detected in:
human liver cancer tissue, human breast cancer tissue, human kidney tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
MCF-7 cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
cystathionase (cystathionine gamma-lyase)

Calculated molecular weight:
405aa,45 kDa

Observed molecular weight:
43-45 kDa,66-70 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CTH

Synonyms
CSE, CTH, Cystathionine gamma lyase, Gamma cystathionase
Background

CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CTH.


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