- Featured Product
- KD/KO Validated
CUL4B Polyclonal antibody
CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12916-1-AP targets CUL4B in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, IF, IHC, IP, RIP, WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, canine |
Immunogen | CUL4B fusion protein Ag3563 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cullin 4B |
Synonyms | CUL 4B, CUL4B, cullin 4B, KIAA0695, MRXHF2, MRXSC, SFM2 |
Calculated Molecular Weight | 913 aa, 104 kDa |
Observed Molecular Weight | 102 kDa |
GenBank Accession Number | BC036216 |
Gene Symbol | CUL4B |
Gene ID (NCBI) | 8450 |
RRID | AB_2086699 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13620 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CUL4B antibody 12916-1-AP | Download protocol |
IF protocol for CUL4B antibody 12916-1-AP | Download protocol |
IP protocol for CUL4B antibody 12916-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
| ||
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
Science CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins. | ||
Nat Med Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.
| ||
Science Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. | ||
Cancer Cell Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase. |