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  • KD/KO Validated

CUL4B Polyclonal antibody

CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12916-1-AP

Print datasheet

Synonyms

CUL 4B, CUL4B, cullin 4B, KIAA0695, MRXHF2, MRXSC, SFM2



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CUL4B Ready-To-Use IHC Kit. CUL4B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12916-1-AP targets CUL4B in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine
Immunogen CUL4B fusion protein Ag3563 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cullin 4B
Synonyms CUL 4B, CUL4B, cullin 4B, KIAA0695, MRXHF2, MRXSC, SFM2
Calculated Molecular Weight 913 aa, 104 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC036216
Gene Symbol CUL4B
Gene ID (NCBI) 8450
RRIDAB_2086699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13620
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

实验方案

Product Specific Protocols
WB protocol for CUL4B antibody 12916-1-APDownload protocol
IF protocol for CUL4B antibody 12916-1-APDownload protocol
IP protocol for CUL4B antibody 12916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP,WB

Cell

FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.

Authors - Jia Z Shen
  • KD Validated
humanWB

Cell

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Authors - Xiulei Mo
humanIHC,IF

Science

CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.

Authors - Chao Yu
humanWB

Nat Med

Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.

Authors - Ruth Eichner
  • KD Validated
humanWB

Science

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Authors - Ting Han
mouseWB

Cancer Cell

Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase.

Authors - Aggarwal Priya P