Back

CX3CL1 Polyclonal antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10108-2-AP

浓度: 1000 μg/ml

点击咨询在线客服

Related Primary Antibodies

Applications

Tested Applications:
IHC, WB,ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IHC, WB


Images See all

WB analysis of PC-3 using 10108-2-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IHC staining of human small intestine using 10108-2-AP

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens)..

IHC staining of human small intestine using 10108-2-AP

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens)..

Species specificity:
human
Positive WB detected in:
PC-3 cells, human brain tissue
Positive IHC detected in:
human small intestine tissue, human lung tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:200-1:800

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CX3CL1 fusion protein Ag0161
Full name:
chemokine (C-X3-C motif) ligand 1
Calculated molecular weight:
42 kDa
Observed molecular weight:
90-100 kDa
GenBank accession number:
BC001163
Gene ID (NCBI):
6376
Gene symbol
CX3CL1
Synonyms
ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

Background

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75kDa precursor and undergoes glycosylation to yield two mature forms: a 100kDa cell membrane bound form and an 85kDa soluble form which is released from the cell surface.The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.

Protocols

PRODUCT SPECIFIC PROTOCOLS

STANDARD PROTOCOLS

Click here to view our Standard Protocols

Back
to top