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CYFIP1/2 Polyclonal antibody

CYFIP1/2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16011-1-AP

Print datasheet

Synonyms

CYFIP1/2, CYFIP2, KIAA1168, p53 inducible protein 121, PIR121

验证数据展示

  • WB analysis of human brain using 16011-1-AP

    WB analysis of human brain using 16011-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16011-1-AP

    WB analysis using 16011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16011-1-AP

    IHC staining of mouse brain using 16011-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16011-1-AP (CYFIP1/2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 16011-1-AP

    IP experiment of mouse brain using 16011-1-AP

    IP result of anti-CYFIP1/2 (IP:16011-1-AP, 4ug; Detection:16011-1-AP 1:700) with mouse brain tissue lysate 4000ug.

  • FC experiment of Jurkat using 16011-1-AP

    FC experiment of Jurkat using 16011-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CYFIP1/2 (16011-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16011-1-AP targets CYFIP1/2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, IP, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CYFIP1/2 fusion protein Ag8749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytoplasmic FMR1 interacting protein 2
Synonyms CYFIP1/2, CYFIP2, KIAA1168, p53 inducible protein 121, PIR121
Calculated Molecular Weight 148 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC011762
Gene Symbol CYFIP2
Gene ID (NCBI) 26999
RRIDAB_2090638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96F07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYFIP1/2 belongs to the CYFIP family and is involved in T-cell adhesion and p53-dependent induction of apoptosis. CYFIP1 contains 1,253 amino acids and shares 88% sequence identity with CYFIP2. CYFIP1 and CYFIP2 are members of a highly conserved protein family and share approximately 99% sequence identity with their mouse orthologs. CYFIP1 has been shown to interact with the small GTPase RAC1, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and RAC1 localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts. This antibody can recognize both CYFIP1 and CYFIP2.

实验方案

Product Specific Protocols
WB protocol for CYFIP1/2 antibody 16011-1-APDownload protocol
IHC protocol for CYFIP1/2 antibody 16011-1-APDownload protocol
IP protocol for CYFIP1/2 antibody 16011-1-APDownload protocol
FC protocol for CYFIP1/2 antibody 16011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dis Markers

Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.

Authors - Nachmany Henny H
View Article
humanWB

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article