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Biotin-conjugated Calretinin Monoclonal antibody

Calretinin Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

IHC

Conjugate

Biotin

CloneNo.

2D7A9

Cat No : Biotin-66496

Print datasheet

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

验证数据展示

  • IHC staining of human brain using Biotin-66496

    IHC staining of human brain using Biotin-66496

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Biotin-66496 (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human brain using Biotin-66496

    IHC staining of human brain using Biotin-66496

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Biotin-66496 (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calretinin antibody included

经过测试的应用

Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-66496 targets Calretinin in IHC applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications IHC
Tested Reactivity Human, mouse, pig, rat
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms 29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol CALB2
Gene ID (NCBI) 794
RRIDAB_2883074
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

实验方案

Product Specific Protocols
IHC protocol for Biotin Calretinin antibody Biotin-66496Download protocol
Standard Protocols
Click here to view our Standard Protocols