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Calsequestrin 2 Polyclonal antibody

Calsequestrin 2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27560-1-AP

Print datasheet

Synonyms

Calsequestrin 2, CASQ2, PDIB2

验证数据展示

  • IP experiment of mouse heart using 27560-1-AP

    IP experiment of mouse heart using 27560-1-AP

    IP result of anti-Calsequestrin 2 (IP:27560-1-AP, 4ug; Detection:27560-1-AP 1:1000) with mouse heart tissue lysate 3720 ug.

  • WB analysis of mouse heart using 27560-1-AP

    WB analysis of mouse heart using 27560-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 27560-1-AP

    IHC staining of human heart using 27560-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 27560-1-AP

    IHC staining of human heart using 27560-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27560-1-AP (Calsequestrin 2 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27560-1-AP targets Calsequestrin 2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen Calsequestrin 2 fusion protein Ag26412 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calsequestrin 2 (cardiac muscle)
Synonyms Calsequestrin 2, CASQ2, PDIB2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC022288
Gene Symbol CASQ2
Gene ID (NCBI) 845
RRIDAB_2880908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14958
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calsequestrin 2 (CASQ2) specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. CASQ2 is an important component of calcium handling as a calcium storage protein in the sarcoplasmic reticulum (SR), playing an important role in the regulation of the cardiac ryanodine receptor (RyR2)-mediated calcium release and SR calcium release refractoriness. Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

实验方案

Product Specific Protocols
WB protocol for Calsequestrin 2 antibody 27560-1-APDownload protocol
IHC protocol for Calsequestrin 2 antibody 27560-1-APDownload protocol
IP protocol for Calsequestrin 2 antibody 27560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols