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Ch-TOG Monoclonal antibody

Ch-TOG Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5G1

Cat No : 67631-1-Ig

Print datasheet

Synonyms

ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

验证数据展示

  • WB analysis using 67631-1-Ig

    WB analysis using 67631-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67631-1-Ig

    IF Staining of HepG2 using 67631-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, HepG2 cells, NCCIT cells, rat brain tissue, mouse brain tissue
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67631-1-Ig targets Ch-TOG in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat, pig
Immunogen Ch-TOG fusion protein Ag28770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoskeleton associated protein 5
Synonyms ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC120869
Gene Symbol CKAP5
Gene ID (NCBI) 9793
RRIDAB_2882832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.

实验方案

Product Specific Protocols
WB protocol for Ch-TOG antibody 67631-1-IgDownload protocol
IF protocol for Ch-TOG antibody 67631-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols