CCAR2 Polyclonal antibody
CCAR2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DBC1/CCAR2 Ready-To-Use IHC Kit. DBC1/CCAR2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 22638-1-AP is CCAR2 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, RIP, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CCAR2 fusion protein Ag18374 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | KIAA1967 |
| Synonyms | ccar2, DBC.1, DBC1, DBC-1, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, Protein KIAA1967 |
| Calculated Molecular Weight | 923 aa, 103 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC018269 |
| Gene Symbol | KIAA1967 |
| Gene ID (NCBI) | 57805 |
| RRID | AB_2879138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N163 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IHC protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IF protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| IP protocol for CCAR2 antibody 22638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''. | ||
Biochem Cell Biol Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1. | ||
Elife Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression |
