DEPDC6/Deptor Polyclonal antibody

DEPDC6/Deptor Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20985-1-AP

Print datasheet

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, DEPDC6/Deptor, Deptor



经过测试的应用

Positive WB detected inHeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEP domain containing 6
Synonyms DEP domain containing 6, DEP.6, DEPDC6, DEPDC6/Deptor, Deptor
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_022783
Gene Symbol DEPDC6
Gene ID (NCBI) 64798
RRIDAB_11182391
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.

实验方案

Product Specific Protocols
WB protocol for DEPDC6/Deptor antibody 20985-1-APDownload protocol
IHC protocol for DEPDC6/Deptor antibody 20985-1-APDownload protocol
IF protocol for DEPDC6/Deptor antibody 20985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Sci Transl Med

The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.

Authors - Fabiana Csukasi
humanWB

Autophagy

TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.

Authors - Yue Zhai
humanWB

Cell Death Differ

NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.

Authors - Hao Li
mouseWB

Oncotarget

Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.

Authors - Mengmeng Cheng
mouseIF

Stem Cell Res Ther

DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.

Authors - Si Chen
mouseWB

Gene

p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.

Authors - Xiaoling Yu