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DIS3L2 Monoclonal antibody

DIS3L2 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat and More (1)

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C9F2

Cat No : 67623-1-Ig

Print datasheet

Synonyms

DIS3 like exonuclease 2, DIS3L2, FAM6A, FLJ36974

验证数据展示

  • FC experiment of Jurkat using 67623-1-Ig

    FC experiment of Jurkat using 67623-1-Ig

    1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human DIS3L2 (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • WB analysis using 67623-1-Ig

    WB analysis using 67623-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, HSC-T6 cells, LNCaP cells, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67623-1-Ig targets DIS3L2 in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications FC, WB, ELISA
Cited Applications WB
Tested Reactivity Human, rat
Cited Reactivityhuman
Immunogen DIS3L2 fusion protein Ag29992 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DIS3 mitotic control homolog (S. cerevisiae)-like 2
Synonyms DIS3 like exonuclease 2, DIS3L2, FAM6A, FLJ36974
Calculated Molecular Weight 885 aa, 99 kDa
Observed Molecular Weight 65 kDa, 99 kda
GenBank Accession NumberBC030113
Gene Symbol DIS3L2
Gene ID (NCBI) 129563
RRIDAB_2882825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IYB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

实验方案

Product Specific Protocols
WB protocol for DIS3L2 antibody 67623-1-IgDownload protocol
FC protocol for DIS3L2 antibody 67623-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential

Authors - Damian M Janecki
View Article