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- KD/KO Validated
DOCK2 Monoclonal antibody
DOCK2 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1D7A8
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, Ramos cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66969-1-Ig targets DOCK2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | DOCK2 fusion protein Ag28582 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dedicator of cytokinesis 2 |
| Synonyms | dedicator of cytokinesis 2, DOCK2, FLJ46592, KIAA0209 |
| Calculated Molecular Weight | 212 kDa, 38 kDa |
| Observed Molecular Weight | 200-210 kDa |
| GenBank Accession Number | BC016996 |
| Gene Symbol | DOCK2 |
| Gene ID (NCBI) | 1794 |
| RRID | AB_2882291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q92608 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DOCK2 antibody 66969-1-Ig | Download protocol |
| IHC protocol for DOCK2 antibody 66969-1-Ig | Download protocol |
| IF protocol for DOCK2 antibody 66969-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.
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