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  • Featured Product
  • KD/KO Validated

DRG1 Polyclonal antibody

DRG1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13190-1-AP

Print datasheet

Synonyms

DRG 1, DRG1, NEDD 3, NEDD3

验证数据展示

  • WB analysis using 13190-1-AP

    WB analysis using 13190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13190-1-AP (DRG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 13190-1-AP

    WB analysis of K-562 using 13190-1-AP

    WB result of DRG1 antibody (13190-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRG1 transfected K-562 cells.

  • IHC staining of human thyroid cancer using 13190-1-AP

    IHC staining of human thyroid cancer using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DRG1 antibody included
  • IP experiment of K-562 using 13190-1-AP

    IP experiment of K-562 using 13190-1-AP

    IP result of anti-DRG1 (IP:13190-1-AP, 4ug; Detection:13190-1-AP 1:600) with K-562 cells lysate 4400 ug.

  • IHC staining of human thyroid cancer using 13190-1-AP

    IHC staining of human thyroid cancer using 13190-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13190-1-AP (DRG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DRG1 antibody included
  • IF Staining of HepG2 using 13190-1-AP

    IF Staining of HepG2 using 13190-1-AP

    Immunofluorescent analysis of HepG2 cells using 13190-1-AP (DRG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, PC-12 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman thyroid cancer tissue, mouse kidney tissue, rat kidney tissue, rat skeletal muscle tissue, human colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DRG1 Ready-To-Use IHC Kit. DRG1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13190-1-AP targets DRG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen DRG1 fusion protein Ag3866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name developmentally regulated GTP binding protein 1
Synonyms DRG 1, DRG1, NEDD 3, NEDD3
Calculated Molecular Weight 367 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC020803
Gene Symbol DRG1
Gene ID (NCBI) 4733
RRIDAB_2095275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y295
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DRG1 antibody 13190-1-APDownload protocol
IHC protocol for DRG1 antibody 13190-1-APDownload protocol
IF protocol for DRG1 antibody 13190-1-APDownload protocol
IP protocol for DRG1 antibody 13190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases.

Authors - Suzana Markolovic
View Article