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DVL2 Monoclonal antibody

DVL2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F2D2

Cat No : 67105-1-Ig

Print datasheet

Synonyms

Dishevelled 2, DSH homolog 2, DVL2

验证数据展示

  • WB analysis using 67105-1-Ig

    WB analysis using 67105-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67105-1-Ig (DVL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 67105-1-Ig

    IHC staining of mouse colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included
  • IHC staining of mouse colon using 67105-1-Ig

    IHC staining of mouse colon using 67105-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67105-1-Ig (DVL2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DVL2 antibody included

经过测试的应用

Positive WB detected inMCF-7 cells, A431 cells, T-47D cells, THP-1 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse colon tissue, human colon cancer tissue, human gliomas tissue, human prostate cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DVL2 Ready-To-Use IHC Kit. DVL2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67105-1-Ig targets DVL2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen DVL2 fusion protein Ag28634 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dishevelled, dsh homolog 2 (Drosophila)
Synonyms Dishevelled 2, DSH homolog 2, DVL2
Calculated Molecular Weight 736 aa, 79 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC014844
Gene Symbol DVL2
Gene ID (NCBI) 1856
RRIDAB_2882409
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14641
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for DVL2 antibody 67105-1-IgDownload protocol
IHC protocol for DVL2 antibody 67105-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols