• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

EAAT2 Monoclonal antibody

EAAT2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C8D10

Cat No : 67083-1-Ig

Print datasheet

Synonyms

EAAT2, GLT 1, GLT1, SLC1A2

验证数据展示

  • WB analysis using 67083-1-Ig

    WB analysis using 67083-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67083-1-Ig (SLC1A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67083-1-Ig

    IHC staining of mouse brain using 67083-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 67083-1-Ig

    IHC staining of rat brain using 67083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 67083-1-Ig

    IHC staining of rat brain using 67083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 67083-1-Ig

    IHC staining of mouse brain using 67083-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67083-1-Ig targets EAAT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen EAAT2 fusion protein Ag19816 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms EAAT2, GLT 1, GLT1, SLC1A2
Calculated Molecular Weight 574 aa, 62 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC132768
Gene Symbol SLC1A2
Gene ID (NCBI) 6506
RRIDAB_2882391
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE.(PMID: 22114258)

实验方案

Product Specific Protocols
WB protocol for EAAT2 antibody 67083-1-IgDownload protocol
IHC protocol for EAAT2 antibody 67083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols