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- KD/KO Validated
EZH2 Polyclonal antibody
EZH2 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
FC, IF/ICC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | DU 145 cells, HEK-293 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Jurkat cells, A431 cells, HepG2 cells, rat kidney tissue, C6 cells |
Positive IP detected in | HepG2 cells |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
This antibody is not recommended for use in IHC.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21800-1-AP targets EZH2 in WB, RIP, IP, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IP, WB, ELISA |
Cited Applications | CHIP, CoIP, IF, IP, RIP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, sheep, pig |
Immunogen | EZH2 fusion protein Ag16789 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | enhancer of zeste homolog 2 (Drosophila) |
Synonyms | Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6 |
Calculated Molecular Weight | 751 aa, 86 kDa |
Observed Molecular Weight | 90-102 kDa |
GenBank Accession Number | BC010858 |
Gene Symbol | EZH2 |
Gene ID (NCBI) | 2146 |
RRID | AB_10858790 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q15910 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for EZH2 antibody 21800-1-AP | Download protocol |
IF protocol for EZH2 antibody 21800-1-AP | Download protocol |
IP protocol for EZH2 antibody 21800-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Struct Mol Biol lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal. | ||
Cancer Res HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc. | ||
Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI
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Nat Commun CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.
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Clin Cancer Res Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.
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