Endoglin/CD105 Monoclonal antibody
Endoglin/CD105 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (3)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2D5E8
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, HUVEC cells, human placenta tissue |
Positive IHC detected in | human breast cancer tissue, human placenta tissue, human renal cell carcinoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue, human liver cancer tissue |
Positive FC detected in | HUVEC cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
Immunofluorescence (IF) | IF : 1:5000-1:20000 |
Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67075-1-Ig targets Endoglin/CD105 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | FC, IF, IHC |
Tested Reactivity | Human |
Cited Reactivity | human, mouse, rat |
Immunogen | Endoglin/CD105 fusion protein Ag27837 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | endoglin |
Synonyms | CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1 |
Calculated Molecular Weight | 71 kDa |
Observed Molecular Weight | 95-100 kDa |
GenBank Accession Number | BC014271 |
Gene Symbol | ENG |
Gene ID (NCBI) | 2022 |
RRID | AB_2882383 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P17813 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
IHC protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
IF protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
FC protocol for Endoglin/CD105 antibody 67075-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Stem Cell Res Ther Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment. | ||
Cell Tissue Res A histological study of vascular wall resident stem cells in venous malformations | ||
Dis Markers Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis | ||
Front Bioeng Biotechnol Quiescence preconditioned nucleus pulposus stem cells alleviate intervertebral disc degeneration by enhancing cell survival via adaptive metabolism pattern in rats |