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FCHO1 Polyclonal antibody

FCHO1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26767-1-AP

Print datasheet

Synonyms

FCH domain only 1, FCH domain only protein 1, FCHO1, KIAA0290

验证数据展示

  • WB analysis of Raji using 26767-1-AP

    WB analysis of Raji using 26767-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26767-1-AP (FCHO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 26767-1-AP

    IHC staining of human tonsillitis using 26767-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26767-1-AP (FCHO1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26767-1-AP is FCHO1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen FCHO1 fusion protein Ag25291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FCH domain only 1
Synonyms FCH domain only 1, FCH domain only protein 1, FCHO1, KIAA0290
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC028021
Gene Symbol FCHO1
Gene ID (NCBI) 23149
RRIDAB_2880626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FCHO1 is composed of 889 amino acid residues and contains an N-terminal FES-CIP4 homology (FCH) domain and an evolutionarily conserved C-terminal FCHO homology (FOH) domain. FCHO1 functions in an early step of clathrin-mediated endocytosis. FCHO1 is required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites of CCV formation (PMID: 20448150).

实验方案

Product Specific Protocols
WB protocol for FCHO1 antibody 26767-1-APDownload protocol
IHC protocol for FCHO1 antibody 26767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols