CoraLite®488-conjugated FUS/TLS Polyclonal antibody

FUS/TLS Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF

Conjugate

CoraLite®488 Fluorescent Dye

Cat No : CL488-11570

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Synonyms

75 kDa DNA pairing protein, FUS, FUS1, Oncogene FUS, Oncogene TLS



经过测试的应用

Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FUS/TLS Ready-To-Use IHC Kit. FUS/TLS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11570-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11570 targets FUS/TLS in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Tested Reactivity human, mouse, rat
Immunogen FUS/TLS fusion protein Ag2150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Synonyms 75 kDa DNA pairing protein, FUS, FUS1, Oncogene FUS, Oncogene TLS
Calculated Molecular Weight 75 kDa
GenBank Accession NumberBC026062
Gene Symbol FUS
Gene ID (NCBI) 2521
RRIDAB_2883079
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35637
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL488 FUS/TLS antibody CL488-11570Download protocol
FC protocol for CL488 FUS/TLS antibody CL488-11570Download protocol
Standard Protocols
Click here to view our Standard Protocols