Flag tag Antibody 20 Publications

Rabbit Polyclonal| Catalog number: 20543-1-AP

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规格

单价

100 μl

¥980

500 μl

¥4000

1000 μl

¥6000


Species specificity:
recombinant protein, mouse

Positive WB detected in:
recombinant protein

Positive IF detected in:
Transfected HEK-293 cells

Recommended dilution:
WB : 1:1000-1:4000
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Flag tag

Calculated molecular weight:

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects FLAG-tagged proteins.


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