DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1D2

Cat No : 60002-1-Ig

Print datasheet

Synonyms

DDDDK tag



经过测试的应用

Positive WB detected inRecombinant Protein
Positive IP detected inRecombinant protein protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60002-1-Ig targets DDDDK tag in WB, IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IP, WB, ELISA
Cited Applications IP, WB
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DDDDK tag
Synonyms DDDDK tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881349
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins.

实验方案

Product Specific Protocols
IP protocol for DDDDK tag antibody 60002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Oncogene

TRIB3 confers radiotherapy resistance in esophageal squamous cell carcinoma by stabilizing TAZ.

Authors - Sha Zhou
WB

Aging (Albany NY)

HOXB9 enhances the ability of lung cancer cells to penetrate the blood-brain barrier.

Authors - HongShan Zheng

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
WB

J Virol

Enterovirus D68 Protease 2Apro Targets TRAF3 to Subvert Host Innate Immune Responses.

Authors - Jun Kang
WB,IP

Apoptosis

Adaptor FADD is recruited by RTN3/HAP in ER-bound signaling complexes.

Authors - Xiang Rong R
WB

Mol Biol Rep

Overexpression of APRIN inhibits differentiation and proliferation and promotes apoptosis in P19 embryonal carcinoma cells.

Authors - Zhou Xiang X