DDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

DDDDK tag Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

2C3F4

Cat No : 66008-1-Ig

Print datasheet

Synonyms

DDDDK tag



Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

产品信息

66008-1-Ig targets DDDDK tag in WB, RIP, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications ELISA
Cited Applications IF, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DDDDK tag
Synonyms DDDDK tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881491
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 2C3F4, which is different from any other vendors.

实验方案

Product Specific Protocols
IF protocol for DDDDK tag antibody 66008-1-IgDownload protocol
IP protocol for DDDDK tag antibody 66008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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Nat Microbiol

Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.

Authors - Hua Zhang
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Cell Metab

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Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
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Plant Cell

Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.

Authors - Cuicui Shen
humanWB

Cell Death Dis

The E3 ligase TRIM26 suppresses ferroptosis through catalyzing K63-linked ubiquitination of GPX4 in glioma

Authors - Zhangjie Wang
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Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong