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G3BP1 Monoclonal antibody

G3BP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

IF, IHC, WB, ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

1E4A2

Cat No : 66486-1-Ig

Print datasheet

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII



经过测试的应用

Positive WB detected inLNCaP cells, RAW 264.7 cells, pig brain tissue, HeLa cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman testis tissue, human colon tissue, human lymphoma tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected insodium arsenite treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66486-1-Ig targets G3BP1 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsCoIP, FC, IF, IP, RIP, WB
Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Immunogen G3BP1 fusion protein Ag3728 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 1
Synonyms G3BP, G3BP 1, G3BP1, HDH VIII
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC006997
Gene Symbol G3BP1
Gene ID (NCBI) 10146
RRIDAB_2819031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13283
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

实验方案

Product Specific Protocols
WB protocol for G3BP1 antibody 66486-1-IgDownload protocol
IHC protocol for G3BP1 antibody 66486-1-IgDownload protocol
IF protocol for G3BP1 antibody 66486-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nature

DDX3X acts as a live-or-die checkpoint in stressed cells by regulating NLRP3 inflammasome.

Authors - Parimal Samir
mouseIF

Nature

Galactosaminogalactan activates the inflammasome to provide host protection.

Authors - Benoit Briard
humanIF

Sci Transl Med

Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy

Authors - Takahiko Nishiyama
mouseIF

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB,IF,IP

Autophagy

Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules

Authors - Cuiwei Yang
humanIF

Nat Chem Biol

K29-linked ubiquitin signaling regulates proteotoxic stress response and cell cycle.

Authors - Yuanyuan Yu