HRP-conjugated GAPDH Antibody 27 Publications

Mouse Monoclonal| Catalog number: HRP-60004

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规格

单价

50 μl

¥720

100 μl

¥1200

500 μl

¥4800

1000 μl

¥7200


Species specificity:
human, mouse, rat, zebrafish, plant, hamsters

Positive WB detected in:
HeLa cells

Recommended dilution:
WB : 1:5000-1:50000

Product Information


Source:
Mouse

Purification method:
Antigen affinity purification

Isotype:
IgG2b

Storage:
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Recombinant Protein

Full name:
glyceraldehyde-3-phosphate dehydrogenase

Calculated molecular weight:
36 kDa

Observed molecular weight:
36 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
GAPDH

Synonyms
G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12
Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


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