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BMP9 Polyclonal antibody

BMP9 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17769-1-AP

Print datasheet

Synonyms

BMP 9, BMP9, BMP-9, Bone morphogenetic protein 9, GDF 2, GDF2

验证数据展示

  • WB analysis of L02 using 17769-1-AP

    WB analysis of L02 using 17769-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17769-1-AP (BMP9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17769-1-AP

    IHC staining of human liver cancer using 17769-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 17769-1-AP

    IHC staining of human liver cancer using 17769-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17769-1-AP (BMP9 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inL02 cells, SMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17769-1-AP is BMP9 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen BMP9 fusion protein Ag12177 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth differentiation factor 2
Synonyms BMP 9, BMP9, BMP-9, Bone morphogenetic protein 9, GDF 2, GDF2
Calculated Molecular Weight 429 aa, 47 kDa
Observed Molecular Weight 30 kDa, 46 kDa
GenBank Accession NumberBC074921
Gene Symbol GDF2
Gene ID (NCBI) 2658
RRIDAB_2878437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UK05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMP9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. Mutations in this gene are associated with hereditary hemorrhagic telangiectasia. Human BMP9 precursor protein contains 429 amino acids and has a calculated molecular mass of about 47 kD. Posttranslational processing results in a mature BMP9 protein of about 13 kD. Once secreted, BMP9 may exist as dimers of mature regions or as BMP9 proregion complexes in which the proregion of BMP9 remains tightly associated with the mature dimer.

实验方案

Product Specific Protocols
WB protocol for BMP9 antibody 17769-1-APDownload protocol
IHC protocol for BMP9 antibody 17769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols