ChromoTek GFP-Booster ATTO594

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

Specificity

GFP and GFP derivates

Applications

IF, IHC, ICC

Conjugate

ATTO 594

Host

Alpaca

Cat No : gba594

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Booster, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

anti-GFP VHH/ Nanobody conjugated to a fluorescent dye for IF/ microscopy.

DescriptionThe GFP-Booster stabilizes, enhances, and reactivates the signal of GFP-fusion proteins. Due to its small size, the GFP-Booster enables higher image quality in epifluorescence, confocal, and super-resolution microscopy:

• Considerably higher tissue penetration rates

• Superior accessibility and labelling of epitopes in crowded cellular/organelle environments

• Less than 2 nm epitope-label displacement minimizes linkage error

• Monovalent VHHs do not cluster their epitopes

• Validation: structure and function characterized

• Consistent and reliable performance due to recombinant production

ApplicationsIF, IHC, ICC
HostAlpaca
Specificity/TargetCFP, AcGFP, EGFP, GFP, GFP S65T, mClover, EGFP A206K, pHluorin, PA-GFP, sfGFP, TagGFP, TagGFP2, Citrine, Ecitrine, EYFP, Venus, YFP, Ypet
For the complete list, please click here: Fluorescent protein specificity table Nano-Booster
ConjugateATTO 594
Physical StateLiquid
Suggested DilutionIF/ICC/IHC: 1:200
Type Nanobody
ClassRecombinant
RRIDAB_2631387
Affinity (KD) Dissociation constant KD of 1 pM
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Store at -20°C/-4°F. Avoid freeze-thaw cycles. Aliquot upon arrival. Protect from light. Stable for 6 months.
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Cell Stem Cell

Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling.

Authors - Mayank Verma
IF

Nat Commun

Transplanted photoreceptor precursors transfer proteins to host photoreceptors by a mechanism of cytoplasmic fusion.

Authors - Mandeep S Singh
IHC

Dev Cell

Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells.

Authors - Davide Gambarotto
IF

EMBO J

A genetic memory initiates the epigenetic loop necessary to preserve centromere position.

Authors - Sebastian Hoffmann
IF

Curr Biol

Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.

Authors - Jeroen Dobbelaere
IF

Proc Natl Acad Sci U S A

Spatial and temporal organization of cadherin in punctate adherens junctions.

Authors - Indrajyoti Indra