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GM2A Monoclonal antibody

GM2A Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E4D3

Cat No : 66080-1-Ig

Print datasheet

Synonyms

Ganglioside GM2 activator, GM2 AP, GM2 ganglioside activator, GM2A, SAP 3

验证数据展示

  • WB analysis of HeLa using 66080-1-Ig

    WB analysis of HeLa using 66080-1-Ig

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells.

  • WB analysis of HeLa using 66080-1-Ig

    WB analysis of HeLa using 66080-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 66080-1-Ig

    FC experiment of HEK-293 using 66080-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHela cells, human placenta tissue
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66080-1-Ig targets GM2A in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, WB, ELISA
Tested Reactivity human
Immunogen GM2A fusion protein Ag4394 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GM2 ganglioside activator
Synonyms Ganglioside GM2 activator, GM2 AP, GM2 ganglioside activator, GM2A, SAP 3
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

实验方案

Product Specific Protocols
WB protocol for GM2A antibody 66080-1-IgDownload protocol
FC protocol for GM2A antibody 66080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols